The extracellular matrix controls stem cell specification and crypt morphology in the developing and adult mouse gut.

ABSTRACT: The rapid renewal of the epithelial gut lining is fuelled by stem cells that reside at the base of intestinal crypts. The signal transduction pathways and morphogens that regulate intestinal stem cell self-renewal and differentiation have been extensively characterised. In contrast, although extracellular matrix (ECM) components form an integral part of the intestinal stem cell niche, their direct influence on the cellular composition is less well understood. We set out to systematically compare the effect of two ECM classes, the interstitial matrix and the basement membrane, on the intestinal epithelium. We found that both collagen I and laminin-containing cultures allow growth of small intestinal epithelial cells with all cell types present in both cultures, albeit at different ratios. T

SUBMITTER: Ramadan R 

PROVIDER: S-EPMC9713296 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature