3D chromatin structure in chondrocytes identifies putative osteoarthritis risk genes.

ABSTRACT: Genome-wide association studies have identified over 100 loci associated with osteoarthritis risk, but the majority of osteoarthritis risk variants are noncoding, making it difficult to identify the impacted genes for further study and therapeutic development. To address this need, we used a multiomic approach and genome editing to identify and functionally characterize potential osteoarthritis risk genes. Computational analysis of genome-wide association studies and ChIP-seq data revealed that chondrocyte regulatory loci are enriched for osteoarthritis risk variants. We constructed a chondrocyte-specific regulatory network by mapping 3D chromatin structure and active enhancers in human chondrocytes. We then intersected these data with our previously collected RNA-seq dataset of chondrocyt

SUBMITTER: Thulson E 

PROVIDER: S-EPMC9713432 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature