Transcriptome analyses in infertile men reveal germ cell-specific expression and splicing patterns.

ABSTRACT: The process of spermatogenesis-when germ cells differentiate into sperm-is tightly regulated, and misregulation in gene expression is likely to be involved in the physiopathology of male infertility. The testis is one of the most transcriptionally rich tissues; nevertheless, the specific gene expression changes occurring during spermatogenesis are not fully understood. To better understand gene expression during spermatogenesis, we generated germ cell-specific whole transcriptome profiles by systematically comparing testicular transcriptomes from tissues in which spermatogenesis is arrested at successive steps of germ cell differentiation. In these comparisons, we found thousands of differentially expressed genes between successive germ cell types of infertility patients. We demonstrate ou

SUBMITTER: Siebert-Kuss LM 

PROVIDER: S-EPMC9713473 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature