Dataset Information

Exploring the Chemical Structures and Phototochemical Properties of Graphene Oxide Derivatives by Laser Desorption/Ionization Time-of-Flight Mass Spectrometry to Develop an Efficient Platform for Mass Spectrometric Analysis.

ABSTRACT: Laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF-MS) analysis is harnessed to investigate the chemical structure and photochemical properties of two distinct graphene oxide (GO) derivatives simultaneously. The GO derivatives are synthesized with modified Brodie's method (BGO) and Hummers' method (HGO) and characterized by LDI-TOF-MS as well as conventional tools. A series of LDI-TOF-MS analyses reveal that BGO provides higher laser energy absorption, photochemical stability, and photothermal conversion property than HGO based on their fragmentation patterns and laser desorption/ionization behavior of a thermometer molecule. Based on these characteristics, BGO exhibits higher efficiency in the LDI-TOF-MS analysis of various small molecules and synthetic polymers than HGO. These different photochemical properties of BGO are derived from its large sp² carbon domains compared to HGO. Based on our findings, the analytical potential of LDI-TOF-MS for GO derivatives is clearly demonstrated, which can be an efficient and unique characterization tool to explore both chemical structures and photochemical properties of various carbon materials.

SUBMITTER: Kim SW

PROVIDER: S-EPMC9713797 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Exploring the Chemical Structures and Phototochemical Properties of Graphene Oxide Derivatives by Laser Desorption/Ionization Time-of-Flight Mass Spectrometry to Develop an Efficient Platform for Mass Spectrometric Analysis.

Kim Seung-Woo SW Cho Joon Young JY Min Kyoung Bin KB Lee Sang-Ryong SR Han Joong Tark JT Kim Young-Kwan YK

ACS omega 20221114 47

Laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF-MS) analysis is harnessed to investigate the chemical structure and photochemical properties of two distinct graphene oxide (GO) derivatives simultaneously. The GO derivatives are synthesized with modified Brodie's method (BGO) and Hummers' method (HGO) and characterized by LDI-TOF-MS as well as conventional tools. A series of LDI-TOF-MS analyses reveal that BGO provides higher laser energy absorption, photochemical stability, ...[more]

PMID: 36467915

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Project description:Streptococcus suis, particularly S. suis serotype 2 (SS2), is an important zoonotic pathogen causing meningitis in humans worldwide. Although the proper classification of the causative and pathogenic serotype is salutary for the clinical diagnosis, cross-reactions leading to the indistinguishability of serotypes by the current serotyping methods are significant limitations. In the present study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of extracted peptides was developed to improve the classification of serotype of S. suis. The peptide mass fingerprint (PMFs) database of S. suis was generated from the whole-cell peptides of 32 reference strains of S. suis isolates obtained from pigs. Thirty-two human S. suis isolates from clinical cases in Thailand were used to validate this alternative serotyping method in direct comparison to the multiplex (m)PCR approach. All reference strains, representing 32 serotypes of S. suis, exhibited their individual PMFs patterns, thus allowing differentiation from one another. Highly pathogenic SS2 and SS14 were clearly differentiated from the otherwise serologically closely related SS1/2 and SS1, respectively. The developed MALDI-TOF-MS serotyping method correctly classified the serotype in 68.8% (22/32) of the same serotype isolates generated from the PMFs database; while the validity for the clinical human isolates was 62.5% (20/32). The agreement between the MALDI-TOF-MS and mPCR serotyping was moderate with a Kappa score of 0.522, considering that mPCR could correctly serotype up to 75%. The present study demonstrated that PMFs from the developed MALDI-TOF-MS-based method could successfully discriminate the previously indistinguishable highly pathogenic SS2 and SS14 from SS1/2 and SS1, respectively. Moreover, this serotyping method distinguished pathogenic SS6, and so is an alternative approach of choice to rapidly and reliably serotype clinically pathogenic S. suis isolates.

Dataset Information

Exploring the Chemical Structures and Phototochemical Properties of Graphene Oxide Derivatives by Laser Desorption/Ionization Time-of-Flight Mass Spectrometry to Develop an Efficient Platform for Mass Spectrometric Analysis.

Publications

Exploring the Chemical Structures and Phototochemical Properties of Graphene Oxide Derivatives by Laser Desorption/Ionization Time-of-Flight Mass Spectrometry to Develop an Efficient Platform for Mass Spectrometric Analysis.

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