Coordinated transcriptional and post-transcriptional epigenetic regulation during skeletal muscle development and growth in pigs.

ABSTRACT:

Background

N6-methyladenosine (m⁶A) and DNA 5-methylcytosine (5mC) methylation plays crucial roles in diverse biological processes, including skeletal muscle development and growth. Recent studies unveiled a potential link between these two systems, implicating the potential mechanism of coordinated transcriptional and post-transcriptional regulation in porcine prenatal myogenesis and postnatal skeletal muscle growth.

Methods

Immunofluorescence and co-IP assays were carried out between the 5mC writers and m⁶A writers to investigate the molecular basis underneath. Large-scale in-house transcriptomic data were compiled for applying weighted correlation network analysis (WGCNA) to identify the co-expression patterns of m⁶A and 5mC regulators and

SUBMITTER: Zhang D 

PROVIDER: S-EPMC9714148 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature