Identifying novel interactions of the colon-cancer related APC protein with Wnt-pathway nuclear transcription factors.

ABSTRACT:

Background

Colon cancer is often driven by mutations of the adenomatous polyposis coli (APC) gene, an essential tumor suppressor gene of the Wnt β-catenin signaling pathway. APC and its cytoplasmic interactions have been well studied. However, various groups have also observed its presence in the nucleus. Identifying novel interactions of APC in the Wnt pathway will provide an opportunity to understand APC's nuclear role better and ultimately identify potential cancer treatment targets.

Methods

We used the all-vs-all sequencing (AVA-Seq) method to interrogate the interactome of protein fragments spanning most of the 60 Wnt β-catenin pathway proteins. Using protein fragments identified the interacting regions between the proteins with more resolution than a full-length protei

SUBMITTER: Al-Thani NM 

PROVIDER: S-EPMC9714242 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature