Point-of-care microchip electrophoresis for integrated anemia and hemoglobin variant testing.

ABSTRACT: Anemia affects over 25% of the world's population with the heaviest burden borne by women and children. Genetic hemoglobin (Hb) variants, such as sickle cell disease, are among the major causes of anemia. Anemia and Hb variant are pathologically interrelated and have an overlapping geographical distribution. We present the first point-of-care (POC) platform to perform both anemia detection and Hb variant identification, using a single paper-based electrophoresis test. Feasibility of this new integrated diagnostic approach is demonstrated via testing individuals with anemia and/or sickle cell disease. Hemoglobin level determination is performed by an artificial neural network (ANN) based machine learning algorithm, which achieves a mean absolute error of 0.55 g dL^-1 and a

SUBMITTER: An R 

PROVIDER: S-EPMC9714341 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature