Knockdown of TMEM160 leads to an increase in reactive oxygen species generation and the induction of the mitochondrial unfolded protein response.

ABSTRACT: Transmembrane protein 160 (TMEM160) was recently reported to be localized to the mitochondrial inner membrane, but mitochondrial function was noted to be unaffected by loss of TMEM160. In contrast to these previously published findings, we report here that the absence of TMEM160 influences intracellular responses. After confirming that TMEM160 is localized in the inner mitochondrial membrane, we knocked down TMEM160 in human cultured cells and analyzed the changes in cellular responses. TMEM160 depletion led to an upregulation of the mitochondrial chaperone HSPD1, suggesting that depletion induced the mitochondrial unfolded protein response (UPR^mt ). Indeed, the expression of key transcription factors that induce the UPR^mt (ATF4, ATF5, and DDIT3) was increased followi

SUBMITTER: Yamashita K 

PROVIDER: S-EPMC9714381 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature