A multi-omics study to characterize the transdifferentiation of human dermal fibroblasts to osteoblast-like cells.

ABSTRACT: Background: Various skeletal disorders display defects in osteoblast development and function. An in vitro model can help to understand underlying disease mechanisms. Currently, access to appropriate starting material for in vitro osteoblastic studies is limited. Native osteoblasts and their progenitors, the bone marrow mesenchymal stem cells, (MSCs) are problematic to isolate from affected patients and challenging to expand in vitro. Human dermal fibroblasts in vitro are a promising substitute source of cells. Method: We developed an in vitro culturing technique to transdifferentiate fibroblasts into osteoblast-like cells. We obtained human fibroblasts from forearm skin biopsy and differentiated them into osteoblast-like cells with ß

SUBMITTER: Pihlstrom S 

PROVIDER: S-EPMC9714459 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature