Energy metabolism in skeletal muscle cells from donors with different body mass index.

ABSTRACT: Obesity and physical inactivity have a profound impact on skeletal muscle metabolism. In the present work, we have investigated differences in protein expression and energy metabolism in primary human skeletal muscle cells established from lean donors (BMI<25 kg/m²) and individuals with obesity (BMI>30 kg/m²). Furthermore, we have studied the effect of fatty acid pretreatment on energy metabolism in myotubes from these donor groups. Alterations in protein expression were investigated using proteomic analysis, and energy metabolism was studied using radiolabeled substrates. Gene Ontology enrichment analysis showed that glycolytic, apoptotic, and hypoxia pathways were upregulated, whereas the pentose phosphate pathway was downregulated in myotubes from donors with obesi

SUBMITTER: Katare PB 

PROVIDER: S-EPMC9714574 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature