Phase I-metabolism studies of the synthetic cannabinoids PX-1 and PX-2 using three different in vitro models.

ABSTRACT:

Purpose

Synthetic cannabinoids (SCs), highly metabolized substances, are rarely found unmodified in urine samples. Urine screening relies on SC metabolite detection, requiring metabolism knowledge. Metabolism data can be acquired via in vitro assays, e.g., human hepatocytes, pooled human liver microsomes (pHLM), cytochrome P450 isoforms and a fungal model; or in vivo by screening, e.g., authentic human samples or rat urine. This work describes the comprehensive study of PX-1 and PX-2 in vitro metabolism using three in vitro models. 5F-APP-PICA (PX-1) and 5F-APP-PINACA (PX-2) were studied as they share structural similarity with AM-2201, THJ-2201 and 5F-AB-PINACA, the metabolism of which was described in the literature.

Methods

For SC incubation, pHLM, cytochrome P450 isoenzy

SUBMITTER: Dahm P 

PROVIDER: S-EPMC9715525 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature