Investigations into photoreceptor energy metabolism during experimental retinal detachment.

ABSTRACT: Retinal detachment is a sight-threatening disorder, which occurs when the photoreceptors are separated from their vascular supply. The aim of the present study was to shed light on photoreceptor energy metabolism during experimental detachment in rats. Retinal detachment was induced in the eyes of rats via subretinal injection of sodium hyaluronate. Initially, we investigated whether detachment caused hypoxia within photoreceptors, as evaluated by the exogenous and endogenous biomarkers pimonidazole and HIF-1α, as well as by qPCR analysis of HIF target genes. The results showed no unequivocal staining for pimonidazole or HIF-1α within any detached retina, nor upregulation of HIF target genes, suggesting that any reduction in pO₂ is of insufficient magnitude to produce hyp

SUBMITTER: Chidlow G 

PROVIDER: S-EPMC9716104 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature