Development of real-time and lateral flow recombinase polymerase amplification assays for rapid detection of Schistosoma mansoni.

ABSTRACT:

Background

Accurate diagnosis followed by timely treatment is an effective strategy for the prevention of complications together with reducing schistosomiasis transmission. Recombinase Polymerase Amplification (RPA) is a simple, rapid, sensitive, and specific isothermal method with low resource needs. This research aimed at the development and optimisation of a real-time (RT) and a lateral flow (LF) RPA assay for the detection of Schistosoma mansoni.

Methodology

Recombinase Polymerase Amplification reactions were performed at full- (as recommended) and half-volumes (to reduce costs), with RT or LF detection systems targeting the S. mansoni mitochondrial minisatellite region. The specificity was assessed using gDNA from other Schistosoma species, h

SUBMITTER: Mesquita SG 

PROVIDER: S-EPMC9716991 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature