Rheumatoid arthritis chondrocytes produce increased levels of pro-inflammatory proteins

ABSTRACT:

Objective

To investigate whether articular chondrocytes from rheumatoid arthritis (RA) patients have acquired a proinflammatory phenotype.

Method

Articular cartilage explants from RA patients and healthy controls (HC) were cultured with or without interleukin (IL)-1β for two weeks. Protein levels of cytokines and metalloproteinases (MMPs) in the supernatant were measured by LUMINEX, mRNA with qPCR and nitrogen oxide (NO) levels with Griess assay.

Results

Within 24 ​h after culture, cartilage explants from RA spontaneously produced MMP-1 and MMP-13, and matrix components (aggrecan and collagen type IV) were released. In addition, the RA explants released higher levels of tumor necrosis factor, interferon-γ, IL-33, IL-18, vascular endothelial growth factor-A, IL-6

SUBMITTER: Onnheim K 

PROVIDER: S-EPMC9718183 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature