CCL2 induces articular chondrocyte MMP expression through ERK and p38 signaling pathways.

ABSTRACT:

Objective

In previous studies, we determined an association between increased serum and articular cartilage levels of CCL2 with osteoarthritis (OA) progression, cartilage damage and increased MMP13 in cartilage. Here we analyzed CCL2 downstream signaling mediators that lead to gene expression of cartilage catabolic markers, in healthy and OA human articular chondrocytes.

Design

Human articular chondrocytes obtained from healthy or OA subjects were treated with or without recombinant human CCL2; cell lysates or mRNA were collected for immunoblotting or qRT-PCR. For pathway analysis, chondrocytes were pre-incubated with an inhibitor of CCR2 (the unique CCL2 receptor), ERK inhibitor or p38 inhibitor prior to CCL2 treatment.

Results

CCL2 treatment of both healthy and OA c

SUBMITTER: Willcockson H 

PROVIDER: S-EPMC9718225 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature