Identification of a cartilage specific novel miRNA which directly targets PRMT3 in rats.

ABSTRACT: Through experiments to testify a candidate novel miRNA previously discovered by us is a real miRNA and involved in cartilage development.

Design

The miR-novel and the newly hairpin miRNA transcribed sequence (pre-miR-novel) was verified as a genuinely existing miRNA by northern blotting. The predicted secondary structure, sequence alignment and targets of pre-miR-novel were performed by "RNAstructure 5.3" program, LASTN2.8.0+/miRbase22 program and RNA hybird program, respective. GO/KEGG pathway analysis also were performed. The miR-novel expression in cartilage tissue during development was detected by RT-qPCR and dot blotting. The chondrocyte differentiation model was established to examine whether miR-novel is involved in cartilage development. T

SUBMITTER: Sun J 

PROVIDER: S-EPMC9718250 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature