Synovial tissue metabolomic profiling reveal biomarkers of synovial inflammation in patients with osteoarthritis.

ABSTRACT:

Objective

Inflammatory responses are associated with changes in tissue metabolism. Prior studies find altered metabolomic profiles in both the synovial fluid (SF) and serum of osteoarthritis subjects. Our study determined the metabolomic profile of synovial tissue (ST) and SF of individuals with osteoarthritis (OA) and its association with synovial inflammation.

Design

37 OA ST samples were collected during joint replacement, 21 also had SF. ST samples were fixed in formalin for histological analysis, cultured (explants) for cytokine analysis by enzyme-linked immunosorbent assay, or snap-frozen for metabolomic analysis. ST samples were categorized by Krenn synovitis score and picrosirius red. CD68 and vimentin expression was assessed by immunohistochemistry and semi-quantifi

SUBMITTER: Murillo-Saich JD 

PROVIDER: S-EPMC9718344 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature