The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice.

ABSTRACT:

Objective

While a role for vitamin K in maintaining joint tissue homeostasis has been proposed based on the presence of vitamin K dependent proteins in cartilage and bone, it is not clear if low vitamin K intake is causally linked to joint tissue degeneration. To address this gap, we manipulated vitamin K status in aging mice to test its effect on age-related changes in articular cartilage and sub-chondral bone.

Methods

Eleven-month old male C57BL6 mice were randomly assigned to a low vitamin K diet containing 120 mcg phylloquinone/kg diet (n = 32) or a control diet containing 1.5 mg phylloquinone/kg diet (n = 30) for 6 months. Knees were evaluated histologically using Safranin O and H&E staining, as well as using micro-CT.

Results

Eleven mice in the low vitamin K die

SUBMITTER: Shea MK 

PROVIDER: S-EPMC9718348 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature