Exogenous laminin exhibits a unique vascular pattern in the brain via binding to dystroglycan and integrins.

ABSTRACT:

Background

Unlike other proteins that exhibit a diffusion pattern after intracerebral injection, laminin displays a vascular pattern. It remains unclear if this unique vascular pattern is caused by laminin-receptor interaction or laminin self-assembly.

Methods

We compared the distribution of various wild-type laminin isoforms in the brain after intracerebral injection. To determine what causes the unique vascular pattern of laminin in the brain, laminin mutants with impaired receptor-binding and/or self-assembly activities and function-blocking antibodies to laminin receptors were used. In addition, the dynamics of laminin distribution and elimination were examined at multiple time points after intracerebral injection.

Results

We found that β2-containing laminins had

SUBMITTER: Ruan J 

PROVIDER: S-EPMC9719645 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature