Molecular characterization of indigenous microbes and its potential as a biological control agent of Fusarium stem rot disease (Fusarium verticillioides) on maize.

ABSTRACT: Fusarium stem rot disease caused by Fusarium verticillioides has become one of the most serious issues confronting Indonesian farmers in recent years. An alternative option for suppressing this disease is to use indigenous microbes as an eco-friendly method to reduce synthetic fungicides. The objective of the research was to identify the molecular characteristics and effectiveness of an indigenous microbial consortium in controlling Fusarium stem rot disease. Identification of indigenous microbes is carried out based on molecular characters using universal primers, namely ITS-1/ITS-4 for fungi and 27F/1492R for bacteria. Nucleotide sequences were analyzed using Bioedit 7.2 version and MEGAX software. In vitro testing was carried out using the dual culture method for in

SUBMITTER: Mirsam H 

PROVIDER: S-EPMC9720014 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature