Tumor Mutational Burden by Whole-Genome Sequencing in Resected NSCLC of Never Smokers.

ABSTRACT:

Background

Data are scarce about tumor mutational burden (TMB) as a biomarker in never smokers with non-small cell lung cancer (NSCLC).

Methods

TMB was assessed by whole-genome sequencing (WGS) and compared with in silico reduced whole-exome sequencing (WES) and targeted commercial next-generation sequencing (NGS) gene panels in 92 paired tumor-normal samples from never smokers who underwent NSCLC resection with curative intent. Analyses were performed to test for association with survival after surgery and to identify the optimal prognostic TMB cutoff.

Results

Tumors of never smokers with NSCLC had low TMB scores (median 1.57 mutations/Mb; range, 0.13-17.94). A TMB cutoff of 1.70 mutations/Mb was associated with a 5-year overall survival of 58% in the high-TMB (42% o

SUBMITTER: Ruel LJ 

PROVIDER: S-EPMC9720425 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature