Performance Comparison of Spectral Distance Calculation Methods.

ABSTRACT: Circular dichroism (CD) spectroscopy is a widely used technique for assessing the higher-order structure (HOS) of biopharmaceuticals, including antibody drugs. Since the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use established quality control guidelines, objective evaluation of spectral similarity has been required in order to assess structural comparability. Several spectral distance quantification methods and weighting functions to increase sensitivity have been proposed, but not many reports have compared their performance for CD spectra. We constructed comparison sets that combine actual spectra and simulated noise and performed a comprehensive performance evaluation of each spectral distance calculation method and weighting functi

SUBMITTER: Oyama T 

PROVIDER: S-EPMC9720711 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature