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Mapping the adult human esophagus in vivo and in vitro.

ABSTRACT: Many esophageal diseases can arise during development or throughout life. Therefore, well-characterized in vitro models and detailed methods are essential for studying human esophageal development, homeostasis and disease. Here, we (1) create an atlas of the cell types observed in the normal adult human esophagus; (2) establish an ancestrally diverse biobank of in vitro esophagus tissue to interrogate homeostasis and injury; and (3) benchmark in vitro models using the adult human esophagus atlas. We created a single-cell RNA sequencing reference atlas using fresh adult esophagus biopsies and a continuously expanding biobank of patient-derived in vitro cultures (n=55 lines). We identify and validate several transcriptionally distinct cell classes in the native human adult esophagus, with four populations belonging to the epithelial layer, including basal, epibasal, early differentiating and terminally differentiated luminal cells. Benchmarking in vitro esophagus cultures to the in vivo reference using single-cell RNA sequencing shows that the basal stem cells are robustly maintained in vitro, and the diversity of epithelial cell types in culture is dependent on cell density. We also demonstrate that cultures can be grown in 2D or as 3D organoids, and these methods can be employed for modeling the complete epithelial layers, thereby enabling in vitro modeling of the human adult esophagus.

SUBMITTER: Ferrer-Torres D

PROVIDER: S-EPMC9720751 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Mapping the adult human esophagus in vivo and in vitro.

Ferrer-Torres Daysha D Wu Joshua H JH Zhang Charles J CJ Hammer Max A MA Dame Michael K MK Wu Angeline A Holloway Emily M EM Karpoff Kateryna K McCarthy Caroline L CL Bohm Margaret S MS Cuttitta Ashley J AJ Tigani Dominic J DJ Huang Sha S Tsai Yu-Hwai YH Miller Alyssa J AJ Walker Taylor T Bayer David E DE Hogan Simon P SP Turgeon Danielle Kim DK Lin Jules J Higgins Peter D R PDR Sexton Jonathan J Spence Jason R JR

Development (Cambridge, England) 20221024 20

Many esophageal diseases can arise during development or throughout life. Therefore, well-characterized in vitro models and detailed methods are essential for studying human esophageal development, homeostasis and disease. Here, we (1) create an atlas of the cell types observed in the normal adult human esophagus; (2) establish an ancestrally diverse biobank of in vitro esophagus tissue to interrogate homeostasis and injury; and (3) benchmark in vitro models using the adult human esophagus atlas ...[more]

PMID: 36278875

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Dataset Information

Mapping the adult human esophagus in vivo and in vitro.

Publications

Mapping the adult human esophagus in vivo and in vitro.

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