P53 immunostaining pattern is a useful surrogate marker for TP53 gene mutations.

ABSTRACT:

Background

TP53 is the most frequently mutated gene in the human cancer, and the awareness of its mutational status is useful in the diagnosis and treatment of cancer patients. In the present study, we investigated the association between TP53 gene mutations and p53 immunohistochemical staining (IHC) patterns and non-genetic effect of MDM2 as a negative regulator of p53.

Methods

A total of 135 solid cancer cases with next generation sequencing data were subjected to p53 IHC and classified as overexpression, null type or usual pattern.

Results

TP53 mutation was observed in 104 out of 135 cases (77.0%). When the TP53 mutations were annotated into DISRUPTED (truncations, frameshifts, splice site mutations, and deep deletions) and IF-DBD (in-frame mutations in the DNA bin

SUBMITTER: Sung YN 

PROVIDER: S-EPMC9720942 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature