Time-resolved microfluidics unravels individual cellular fates during double-strand break repair.

ABSTRACT:

Background

Double-strand break repair (DSBR) is a highly regulated process involving dozens of proteins acting in a defined order to repair a DNA lesion that is fatal for any living cell. Model organisms such as Saccharomyces cerevisiae have been used to study the mechanisms underlying DSBR, including factors influencing its efficiency such as the presence of distinct combinations of microsatellites and endonucleases, mainly by bulk analysis of millions of cells undergoing repair of a broken chromosome. Here, we use a microfluidic device to demonstrate in yeast that DSBR may be studied at a single-cell level in a time-resolved manner, on a large number of independent lineages undergoing repair.

Results

We used engineered S. cerevisiae cells in which GFP is expressed followin

SUBMITTER: Vertti-Quintero N 

PROVIDER: S-EPMC9720956 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature