Cultivation-independent genomes greatly expand taxonomic-profiling capabilities of mOTUs across various environments.

ABSTRACT:

Background

Taxonomic profiling is a fundamental task in microbiome research that aims to detect and quantify the relative abundance of microorganisms in biological samples. Available methods using shotgun metagenomic data generally depend on the deposition of sequenced and taxonomically annotated genomes, usually from cultures of isolated strains, in reference databases (reference genomes). However, the majority of microorganisms have not been cultured yet. Thus, a substantial fraction of microbial community members remains unaccounted for during taxonomic profiling, particularly in samples from underexplored environments. To address this issue, we developed the mOTU profiler, a tool that enables reference genome-independent species-level profiling of metagenomes. As such, it suppo

SUBMITTER: Ruscheweyh HJ 

PROVIDER: S-EPMC9721005 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature