Establishment of indirect ELISA method for Salmonella antibody detection from ducks based on PagN protein.

ABSTRACT:

Background

Salmonella as an important food-borne zoonotic bacterial pathogen, infection in ducks is a recessive infection, however, it can also cause high mortality and threat to food safety. Preventing and controlling the infection and transmission of Salmonella in ducks critically require rapid and sensitive detection method. Full-length Salmonella-specific protein PagN was induced and expressed in E.coil BL21 and was purified as an antigen to establish an indirect enzyme-linked immunosorbent assays (iELSA) detection kit.

Results

The recombinant PagN protein has a molecular weight of 43 kDa containing a His-tag, was recognized by an anti-Salmonella positive serum by Western blot assay. The optimal concentration of PagN as a coating antigen in the iELISA was 1 μg/mL, and th

SUBMITTER: Hou S 

PROVIDER: S-EPMC9721058 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature