Molecular detection and characterisation of the first Japanese encephalitis virus belonging to genotype IV acquired in Australia.

ABSTRACT:

Background

A fatal case of Japanese encephalitis (JE) occurred in a resident of the Tiwi Islands, in the Northern Territory of Australia in February 2021, preceding the large JE outbreak in south-eastern Australia in 2022. This study reports the detection, whole genome sequencing and analysis of the virus responsible (designated JEV/Australia/NT_Tiwi Islands/2021).

Methods

Reverse transcription quantitative PCR (RT-qPCR) testing was performed on post-mortem brain specimens using a range of JE virus (JEV)-specific assays. Virus isolation from brain specimens was attempted by inoculation of mosquito and mammalian cells or embryonated chicken eggs. Whole genome sequencing was undertaken using a combination of Illumina next generation sequencing methodologies, including a tiling

SUBMITTER: Sikazwe C 

PROVIDER: S-EPMC9721490 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature