Analysis of lytic polysaccharide monooxygenase activity in thermophilic fungi by high-performance liquid chromatography-refractive index detector.

ABSTRACT:

Introduction

Most current methods for analysing the activity of LPMO are based on the quantification of H₂O₂, a side product of LPMO; however, these methods cannot assay the LPMO activity of thermophilic fungi because of the low thermostability of H₂O₂. Therefore, we present a high-performance liquid chromatography-refractive index detector (HPLC-RID) method to assay the LPMO activity of the thermophilic fungus Thermoascus aurantiacus.

Results

According to the established method, the specific activities of nTaAA9A C1 and C4 oxidation were successfully analysed and were 0.646 and 0.574 U/mg, respectively. By using these methods, we analyzed the C1 and C4 oxidation activities of the recombinant TaAA9A (rTaAA9A) and mutated rTa

SUBMITTER: Yu W 

PROVIDER: S-EPMC9722967 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature