Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions.

ABSTRACT: Bone tissue features a complex microarchitecture and biomolecular composition, which determine biomechanical properties. In addition to state-of-the-art technologies, innovative optical approaches allowing the characterization of the bone in native, label-free conditions can provide new, multi-level insight into this inherently challenging tissue. Here, we exploited multimodal nonlinear optical (NLO) microscopy, including co-registered stimulated Raman scattering, two-photon excited fluorescence, and second-harmonic generation, to image entire vertebrae of murine spine sections. The quantitative nature of these nonlinear interactions allowed us to extract accurate biochemical, morphological, and topological information on the bone tissue and to highlight differences between normal and path

SUBMITTER: Talone B 

PROVIDER: S-EPMC9723390 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature