Validation of the application of gel beads-based single-cell genome sequencing platform to soil and seawater.

ABSTRACT: Single-cell genomics is applied to environmental samples as a method to solve the problems of current metagenomics. However, in the fluorescence-activated cell sorting-based cell isolation and subsequent whole genome amplification, the sorting efficiency and the sequence quality are greatly affected by the type of target environment, limiting its adaptability. Here, we developed an improved single-cell genomics platform, named SAG-gel, which utilizes gel beads for single-cell isolation, lysis, and whole genome amplification. To validate the versatility of SAG-gel, single-cell genome sequencing was performed with model bacteria and microbial samples collected from eight environmental sites, including soil and seawater. Gel beads enabled multiple lysis treatments. The genome coverage with mo

SUBMITTER: Nishikawa Y 

PROVIDER: S-EPMC9723564 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature