High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITS.

ABSTRACT: Microbial communities are inter-connected systems of incredible complexity and dynamism that play crucial roles in health, energy, and the environment. To better understand microbial communities and how they respond to change, it is important to know which microbes are present and their relative abundances at the greatest taxonomic resolution possible. Here, we describe a novel protocol (RoC-ITS) that uses the single-molecule Nanopore sequencing platform to assay the composition of microbial communities at the subspecies designation. Using rolling-circle amplification, this methodology produces long-read sequences from a circular construct containing the complete 16S ribosomal gene and the neighboring internally transcribed spacer (ITS). These long reads can be used to generate a high-fide

SUBMITTER: Rusch DB 

PROVIDER: S-EPMC9723582 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature