Long-read single-molecule RNA structure sequencing using nanopore.

ABSTRACT: RNA molecules can form secondary and tertiary structures that can regulate their localization and function. Using enzymatic or chemical probing together with high-throughput sequencing, secondary structure can be mapped across the entire transcriptome. However, a limiting factor is that only population averages can be obtained since each read is an independent measurement. Although long-read sequencing has recently been used to determine RNA structure, these methods still used aggregate signals across the strands to detect structure. Averaging across the population also means that only limited information about structural heterogeneity across molecules or dependencies within each molecule can be obtained. Here, we present Single-Molecule Structure sequencing (SMS-seq) that combines structu

SUBMITTER: Bizuayehu TT 

PROVIDER: S-EPMC9723614 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature