Estrogen regulates divergent transcriptional and epigenetic cell states in breast cancer.

ABSTRACT: Breast cancers are known to be driven by the transcription factor estrogen receptor and its ligand estrogen. While the receptor's cis-binding elements are known to vary between tumors, heterogeneity of hormone signaling at a single-cell level is unknown. In this study, we systematically tracked estrogen response across time at a single-cell level in multiple cell line and organoid models. To accurately model these changes, we developed a computational tool (TITAN) that quantifies signaling gradients in single-cell datasets. Using this approach, we found that gene expression response to estrogen is non-uniform, with distinct cell groups expressing divergent transcriptional networks. Pathway analysis suggested the two most distinct signatures are driven separately by ER and FOXM1. We observe

SUBMITTER: Ors A 

PROVIDER: S-EPMC9723652 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature