Morphine and high-fat diet differentially alter the gut microbiota composition and metabolic function in lean versus obese mice.

ABSTRACT: There are known associations between opioids, obesity, and the gut microbiome, but the molecular connection/mediation of these relationships is not understood. To better clarify the interplay of physiological, genetic, and microbial factors, this study investigated the microbiome and host inflammatory responses to chronic opioid administration in genetically obese, diet-induced obese, and lean mice. Samples of feces, urine, colon tissue, and plasma were analyzed using targeted LC-MS/MS quantification of metabolites, immunoassays of inflammatory cytokine levels, genome-resolved metagenomics, and metaproteomics. Genetic obesity, diet-induced obesity, and morphine treatment in lean mice each showed increases in distinct inflammatory cytokines. Metagenomic assembly and binning uncovered over 4

SUBMITTER: Blakeley-Ruiz JA 

PROVIDER: S-EPMC9723762 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature