A functional loop between YTH domain family protein YTHDF3 mediated m⁶A modification and phosphofructokinase PFKL in glycolysis of hepatocellular carcinoma.

ABSTRACT:

Background & aims

N⁶-methyladenosine (m⁶A) modification plays a critical role in progression of hepatocellular carcinoma (HCC), and aerobic glycolysis is a hallmark of cancer including HCC. However, the role of YTHDF3, one member of the core readers of the m⁶A pathway, in aerobic glycolysis and progression of HCC is still unclear.

Methods

Expression levels of YTHDF3 in carcinoma and surrounding tissues of HCC patients were evaluated by immunohistochemistry. Loss and gain-of-function experiments in vitro and in vivo were used to assess the effects of YTHDF3 on HCC cell proliferation, migration and invasion. The role of YTHDF3 in hepatocarcinogenesis was observed in a chemically induced HCC model with Ythdf3^-/- mice. Untargeted metab

SUBMITTER: Zhou R 

PROVIDER: S-EPMC9724358 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature