Virulence-related gene wx2 of Toxoplasma gondii regulated host immune response via classic pyroptosis pathway.

ABSTRACT:

Background

Toxoplasma gondii is known as the most successful parasite, which can regulate the host immune response through a variety of ways to achieve immune escape. We previously reported that a novel gene wx2 of T. gondii may be a virulence-related molecule. The objective of this study was to explore the mechanism of wx2 regulating host immune response.

Methods

The wx2 knockout strain (RH^wx2-/- strain) and complementary strain (RH^wx2+/+ strain) were constructed by the CRISPR/Cas9 technique, and the virulence of the wx2 gene was detected and changes in pyroptosis-related molecules were observed.

Results

Compared with the wild RH and RH^wx2+/+ strain groups, the survival time for mice infected with the RH^wx2-/- strain was pr

SUBMITTER: Ma Z 

PROVIDER: S-EPMC9724370 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature