An Engineered B. subtilis Inducible Promoter System with over 10 000-Fold Dynamic Range.

ABSTRACT: Bacillus subtilis is the leading model Gram-positive bacterium, and a widely used chassis for industrial protein production. However, B. subtilis research is limited by a lack of inducible promoter systems with low leakiness and high dynamic range. Here, we engineer an inducible promoter system based on the T7 RNA Polymerase (T7 RNAP), the lactose repressor LacI, and the chimeric promoter P_T7lac, integrated as a single copy in the B. subtilis genome. In the absence of IPTG, LacI strongly represses T7 RNAP and P_T7lac and minimizes leakiness. Addition of IPTG derepresses P_T7lac and simultaneously induces expression of T7RNAP, which results in very high output expression. Using green fluorescent and β-galactosidase rep

SUBMITTER: Castillo-Hair SM 

PROVIDER: S-EPMC9724499 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature