Construction of a density mutant collection in bitter gourd via new germplasms innovation and gene functional study.

ABSTRACT: Although a few studies have elucidated the creation of bitter gourd mutants, the suitable concentration and duration of ethyl methanesulfonate (EMS) mutagenesis have not been determined. In this study, mutant collection was conducted to create new germplasms and widen genetic diversity. By employing the seeds of the inbred line Y52 as the mutagenic material, EMS as the mutagen, and the suitable mutagenic conditions for bitter gourd seeds (EMS concentration 0.2%, mutagenic time 10 h), we mutated 10,000 seeds and acquired 3223 independent M₁ lines. For the randomly selected 1000 M₂ lines, 199 M₂ lines with visible phenotypes were found, and 167 M₂ lines were mutants of fruit shape, size, and tubercles. Furthermore, fourteen dwarf, eleven leaf color

SUBMITTER: Yu R 

PROVIDER: S-EPMC9724616 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature