Snapshot imprinting as a tool for surface mapping and identification of novel biomarkers of senescent cells.

ABSTRACT: Cellular senescence has proved to be a strong contributor to ageing and age-related diseases, such as cancer and atherosclerosis. Therefore, the protein content of senescent cells is highly relevant to drug discovery, diagnostics and therapeutic applications. However, current technologies for the analysis of proteins are based on a combination of separation techniques and mass spectrometry, which require handling large sample sizes and a large volume of data and are time-consuming. This limits their application in personalised medicine. An easy, quick and inexpensive procedure is needed for qualitative and quantitative analysis of proteins expressed by a cell or tissue. Here, we describe the use of the "snapshot imprinting" approach for the identification of proteins differentially express

SUBMITTER: Piletska E 

PROVIDER: S-EPMC9724690 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature