A novel interaction between extracellular vimentin and fibrinogen in fibrin formation.

ABSTRACT:

Introduction

Thrombosis is frequently manifested in critically ill patients with systemic inflammation, including sepsis and COVID-19. The coagulopathy in systemic inflammation is often associated with increased levels of fibrinogen and D-dimer. Because elevated levels of vimentin have been detected in sepsis, we sought to investigate the relationship between vimentin and the increased fibrin formation potential observed in these patients.

Materials and methods

This hypothesis was examined by using recombinant human vimentin, anti-vimentin antibodies, plasma derived from healthy and critically ill patients, confocal microscopy, co-immunoprecipitation assays, and size exclusion chromatography.

Results

The level of vimentin in plasma derived from critically ill subjects

SUBMITTER: Martinez-Vargas M 

PROVIDER: S-EPMC9726209 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature