Composition of Sexual Fluids in Cycas revoluta Ovules During Pollination and Fertilization.

ABSTRACT: The composition of fluids that mediate fertilization in cycads is described for the first time. Using tandem mass spectrometry, proteomes of two stages of fluid production, megagametophyte fluid and archegonial chamber fluid production, are compared in Cycas revoluta. These were compared with the proteome of another sexual fluid produced by ovules, the pollination drop proteins. Cycad ovules produce complex liquids immediately prior fertilization. Compared with the pollination drops that mainly had few proteins in classes involved in defense and carbohydrate modification, megagametophyte fluid and archegonial chamber fluid had larger proteomes with many more protein classes, e.g. proteins involved in programmed cell death. Using high-performance liquid chromatography, megagametophyt

SUBMITTER: von Aderkas P 

PROVIDER: S-EPMC9726676 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature