Does filter-aided sample preparation provide sufficient method linearity for quantitative plant shotgun proteomics?

ABSTRACT: Due to its outstanding throughput and analytical resolution, gel-free LC-based shotgun proteomics represents the gold standard of proteome analysis. Thereby, the efficiency of sample preparation dramatically affects the correctness and reliability of protein quantification. Thus, the steps of protein isolation, solubilization, and proteolysis represent the principal bottleneck of shotgun proteomics. The desired performance of the sample preparation protocols can be achieved by the application of detergents. However, these compounds ultimately compromise reverse-phase chromatographic separation and disrupt electrospray ionization. Filter-aided sample preparation (FASP) represents an elegant approach to overcome these limitations. Although this method is comprehensively validated for cell pr

SUBMITTER: Leonova T 

PROVIDER: S-EPMC9728026 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature