Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer.

ABSTRACT: Highly regenerative tissues continuously produce terminally differentiated cells to replace those that are lost. How they orchestrate the complex transition from undifferentiated stem cells towards post-mitotic, molecularly distinct and often spatially segregated differentiated populations is not well understood. In the adult skin epidermis, the stem cell compartment contains molecularly heterogeneous subpopulations^1-4 whose relationship to the complete trajectory of differentiation remains unknown. Here we show that differentiation, from commitment to exit from the stem cell layer, is a multi-day process wherein cells transit through a continuum of transcriptional changes with upregulation of differentiation genes preceding downregulation of typical stemness genes. Differentiat

SUBMITTER: Cockburn K 

PROVIDER: S-EPMC9729105 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature