In vivo assessment of triazine lipid nanoparticles as transfection agents for plasmid DNA.

ABSTRACT: Non-viral vectors for in vivo delivery of plasmid DNA rely on optimized formulations to achieve robust transgene expression. Several cationic lipids have been developed to deliver nucleic acids, but most recent literature has focused on mRNA due to its increased expression profile and excluded plasmid DNA, which may have the advantage of being less immunogenic. In this study, we describe the in vivo evaluation of cationic triazine based lipids, previously prepared by our group. We identify one lipid with limited in vivo toxicity for studies to optimize the lipid formulations, which include an evaluation of the influence of PEG and helper lipids on transgene expression. We then demonstrate that lipoplexes, but not lipid nanoparticles, formed from triazine lipids achieve

SUBMITTER: Nardo D 

PROVIDER: S-EPMC9729407 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature