Methyltransferase-like 3 modulates visceral hypersensitivity through regulating the nuclear export of circKcnk9 in YTHDC1-dependent manner.

ABSTRACT: Background: Accumulating evidence shows that N6-methyladenosine (m⁶A) modulators contribute to the process of chronic pain. However, the exact mechanisms of m⁶A writers involved in visceral hypersensitivity of Irritable bowel syndrome (IBS) remain unclear. This article aimed to reveal a new mechanism for the progression of IBS. Methods: The IBS-like model was established by neonatal colorectal distention (CRD). The relationship between m⁶A and circKcnk9 was analyzed by bioinformatics, immunofluorescence and RNA fluorescence in situ hybridization (FISH) assays. Visceral hypersensitivity was assessed based on the electromyography (EMG) response of the abdominal external oblique muscle to CRD. In vivo and in vitro studies (including EMG st

SUBMITTER: Lin W 

PROVIDER: S-EPMC9730012 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature