METTL3 promotes glycolysis and cholangiocarcinoma progression by mediating the m6A modification of AKR1B10.

ABSTRACT:

Objective

N6-methyladenosine (m6A) RNA methylation is involved in governing the mechanism of tumor progression. We aimed to excavate the biological role and mechanism of the m6A methyltransferase METTL3 in cholangiocarcinoma (CCA).

Methods

METTL3 expression was determined by database and tissue microarray analyses. The role of METTL3 in CCA was explored by loss- and gain-of-function experiments. The m6A target of METTL3 was detected by RNA sequencing. The role of AKR1B10 in CCA was explored, and the association between METTL3 and AKR1B10 was confirmed by rescue experiments.

Result

METTL3 expression was upregulated in CCA tissue, and higher METTL3 expression was implicated in poor prognoses in CCA patients. Overexpression of METTL3 facilitated proliferation, migration,

SUBMITTER: Cai J 

PROVIDER: S-EPMC9730622 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature