Persistent epigenetic alterations in transcription factors after a sustained virological response in hepatocellular carcinoma.

ABSTRACT:

Background and aim

The risk of hepatocellular carcinoma (HCC) persists in a condition of sustained virologic response (SVR) after hepatitis C virus (HCV) eradication. Comprehensive molecular analyses were performed to test the hypothesis that epigenetic abnormalities present after an SVR play a role in hepatocarcinogenesis.

Methods

Whole-genome methylome and RNA sequencing were performed on HCV, SVR, and healthy liver tissue. Integrated analysis of the sequencing data focused on expression changes in transcription factors and their target genes, commonly found in HCV and SVR. Identified expression changes were validated in demethylated cultured HCC cell lines and an independent validation cohort.

Results

The coincidence rates of the differentially methylated regions b

SUBMITTER: Sugimachi K 

PROVIDER: S-EPMC9730721 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature