Fluorescence Spectroscopic Analysis of Lateral and Transbilayer Fluidity of Exosome Membranes.

ABSTRACT: Exosomes are small extracellular vesicles (sEVs) involved in distal cell-cell communication and cancer migration by transferring functional cargo molecules. Membrane domains similar to lipid rafts are assumed to occur in exosome membranes and are involved in interactions with target cells. However, the bilayer membrane properties of these small vesicles have not been fully investigated. Therefore, we examined the fluidity, lateral domain separation, and transbilayer asymmetry of exosome membranes using fluorescence spectroscopy. Although there were some differences between the exosomes, TMA-DPH anisotropy showing moderate lipid chain order indicated that ordered phases comprised a significant proportion of exosome membranes. Selective TEMPO quenching of the TMA-DPH fluorescence in the liqu

SUBMITTER: Yasuda T 

PROVIDER: S-EPMC9731264 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature