Expansion of CD8+ T cell population in Lassa virus survivors with low T cell precursor frequency reveals durable immune response in most survivors.

ABSTRACT:

Introduction

Lassa virus is a priority pathogen for vaccine research and development, however the duration of cellular immunity and protection in Lassa fever (LF) survivors remains unclear.

Methods

We investigated Lassa virus specific CD8+ T cell responses in 93 LF survivors. Peripheral blood mononuclear cells from these individuals were infected with recombinant vesicular stomatitis virus encoding Lassa virus antigens and virus specific T cell responses were measured after 18-hour incubation. Participants who had undetectable CD8+ T cell response underwent further analysis using a 10-day T cell proliferation assays to evaluate for low T cell precursor frequency.

Results

Forty-five of the 93 LF survivors did not have a Lassa virus specific CD8+ T cell response. Of tho

SUBMITTER: LaVergne SM 

PROVIDER: S-EPMC9731491 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature